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With regard to the strategies useful for prevention of possible muscle loss related to the use of second-generation incretin analogs (semaglutide and tirzepatide), preliminary evidence suggests that individuals taking these medications should aim for a daily protein intake ≥1.2 g/kg actual body weight and for physical activity programs including resistance training exercises (one to three sets of 8–12 repetitions for each major muscle group) at least twice a week [216]

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Analysis of these endpoints was carried out using an ImageJ-based method (available online: Time interval analysis was done using the Time Series Analyzer V3 plug-in (available online: for ImageJ to analyze the pattern of change in the dynamic pixel intensity over time